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sonication cell lysis buffer  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc sonication cell lysis buffer
    Sonication Cell Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sonication+buffer/bio_rxiv__64898__2026__04__03__715913-328-7-11
    Average 86 stars, based on 1 article reviews
    sonication cell lysis buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Chromatin Immunoprecipitation:

    Article Title: Antitumor and modeling studies of a penetratin-peptide that targets E2F-1 in small cell lung cancer
    Article Snippet: Cells were washed with cold phosphate-buffered saline and resuspended in PBS containing protease inhibitors, pelleted by centrifugation, resuspended in buffer B (Cell Signaling) with Micrococcal Nuclease and incubated at 37 °C for 20 min and nuclease activity stopped by addition of EDTA. .. Cell nuclei were pelleted, washed once in ChIP buffer (Cell Signaling) incubated for 10 min on ice and resuspended in sonication buffer with protease inhibitors (Cell Signaling). ..

    Incubation:

    Article Title: Antitumor and modeling studies of a penetratin-peptide that targets E2F-1 in small cell lung cancer
    Article Snippet: Cells were washed with cold phosphate-buffered saline and resuspended in PBS containing protease inhibitors, pelleted by centrifugation, resuspended in buffer B (Cell Signaling) with Micrococcal Nuclease and incubated at 37 °C for 20 min and nuclease activity stopped by addition of EDTA. .. Cell nuclei were pelleted, washed once in ChIP buffer (Cell Signaling) incubated for 10 min on ice and resuspended in sonication buffer with protease inhibitors (Cell Signaling). ..

    Sonication:

    Article Title: Antitumor and modeling studies of a penetratin-peptide that targets E2F-1 in small cell lung cancer
    Article Snippet: Cells were washed with cold phosphate-buffered saline and resuspended in PBS containing protease inhibitors, pelleted by centrifugation, resuspended in buffer B (Cell Signaling) with Micrococcal Nuclease and incubated at 37 °C for 20 min and nuclease activity stopped by addition of EDTA. .. Cell nuclei were pelleted, washed once in ChIP buffer (Cell Signaling) incubated for 10 min on ice and resuspended in sonication buffer with protease inhibitors (Cell Signaling). ..

    Article Title: Chromatin accessibility landscape of relapsed pediatric B-lineage acute lymphoblastic leukemia.
    Article Snippet: .. Chromatin pellets obtained by centrifugation at 1700 × g for 5min were washed twice with 300μl sonication buffer [10mM Tris-HCl pH 8.0, 1mM EDTA pH 8.0, 0.1% SDS, 3μl Protease/Phosphatase Inhibitor Cocktail (CST, 5872)] and resuspended with 120μl sonication buffer in microTUBE, followed by sonicationwith CovarisM220 sonicator for 15min at 7 °C until the size of most fragments was in the range of 200–700 bp. .. Sonicated chromatin was rotated at 4 °C for 2 h with 5μl of anti-histone H3K27ac antibody (Abcam, 4729), 2 μl spike-in antibody (Active motif, 61686) and 5μl spike-in chromatin (Active motif, 53083).

    Article Title: Chromatin accessibility landscape of relapsed pediatric B-lineage acute lymphoblastic leukemia
    Article Snippet: .. Chromatin pellets obtained by centrifugation at 1700 × g for 5 min were washed twice with 300 μl sonication buffer [10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0, 0.1% SDS, 3 μl Protease/Phosphatase Inhibitor Cocktail (CST, 5872)] and resuspended with 120 μl sonication buffer in microTUBE, followed by sonication with Covaris M220 sonicator for 15 min at 7 °C until the size of most fragments was in the range of 200–700 bp. .. Sonicated chromatin was rotated at 4 °C for 2 h with 5 μl of anti-histone H3K27ac antibody (Abcam, 4729), 2 μl spike-in antibody (Active motif, 61686) and 5 μl spike-in chromatin (Active motif, 53083).

    Centrifugation:

    Article Title: Chromatin accessibility landscape of relapsed pediatric B-lineage acute lymphoblastic leukemia.
    Article Snippet: .. Chromatin pellets obtained by centrifugation at 1700 × g for 5min were washed twice with 300μl sonication buffer [10mM Tris-HCl pH 8.0, 1mM EDTA pH 8.0, 0.1% SDS, 3μl Protease/Phosphatase Inhibitor Cocktail (CST, 5872)] and resuspended with 120μl sonication buffer in microTUBE, followed by sonicationwith CovarisM220 sonicator for 15min at 7 °C until the size of most fragments was in the range of 200–700 bp. .. Sonicated chromatin was rotated at 4 °C for 2 h with 5μl of anti-histone H3K27ac antibody (Abcam, 4729), 2 μl spike-in antibody (Active motif, 61686) and 5μl spike-in chromatin (Active motif, 53083).

    Article Title: Chromatin accessibility landscape of relapsed pediatric B-lineage acute lymphoblastic leukemia
    Article Snippet: .. Chromatin pellets obtained by centrifugation at 1700 × g for 5 min were washed twice with 300 μl sonication buffer [10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0, 0.1% SDS, 3 μl Protease/Phosphatase Inhibitor Cocktail (CST, 5872)] and resuspended with 120 μl sonication buffer in microTUBE, followed by sonication with Covaris M220 sonicator for 15 min at 7 °C until the size of most fragments was in the range of 200–700 bp. .. Sonicated chromatin was rotated at 4 °C for 2 h with 5 μl of anti-histone H3K27ac antibody (Abcam, 4729), 2 μl spike-in antibody (Active motif, 61686) and 5 μl spike-in chromatin (Active motif, 53083).



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